به جهت کار با دستگاه مخلوط را درلوله های فالکون داخل دستگاه قرار میدهند طوریکه با چرخش دستگاه به سمت خارج از مرکز لوله ها به حالت افقی قرار میگیرند. در اینحالت نیروی گریز از مرکز میخواهد که مخلوط را برخلاف مرکز سانتریفیوژ براند و از این نقطه دور کند و ذرات یا مایع سنگین تر بیش تر به سمت بیرون (یا ته مخلوط) رانده میشوند.
وقتی سانتریفوژ از حرکت بازمی‌ایستد مواد به همین حالت غیر مخلوط می‌مانند. خون و سایر نمونه‌های بیولوژیکی را معمولاً به وسیله دستگاه سانتریفیوژ جدا میکنند.
سانتریفیوژ فالکون در تعداد شاخه های مختلف و سایزهای مختلف قابل اراته میباشد.
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